South Asian Research Publishing Organization (SARPO)

Lubna Shakir^,; Sanam Asi^⸶; Ayazul Haq; Sajjadul Haq; Khan Zada; Muhammad Said; Muhammad Sajid; Shakir Ullah*Rihan Ullah.

Both authors equally contributed to this study

State Key Laboratory of Systematic and Evolutionary Botany (LSEB) Institute of Botany Chines Academy of Science China

Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi Pakistan

Department of chemistry, Govt Ghazi Umara Khan Degree College Samarbagh Dir Lower Khyber Pakhtoonkhwa Pakistan

Department of Botany, Bacha Khan University, Charsadda, Khyber Pakhtoonkhwa Pakistan, Pakistan

INTRODUCTION

Phytochemicals (from the Greek word Phyto, meaning plant) are biologically active, naturally occurring chemical compounds found in plants, which provide health benefits for humans further than those attributed to macronutrients and micronutrients (Hasler and Blumberg, 1999).

They protect plants from disease and damage and contribute to the plant’s color, aroma, and flavor. In general, the plant chemicals that protect plant cells from environmental hazards such as pollution, stress, drought, UV exposure, and pathogenic attack are called phytochemicals (Ullah et al. 2023).

Recently, it is clearly known that they have roles in the protection of human health when their dietary intake is significant. More than 3,000 phytochemicals have been cataloged and classified by protective function, physical characteristics, and chemical characteristics and About 150 phytochemicals have been studied in detail (Ullah et al., 2021). Wide-ranging dietary phytochemicals are found in fruits, vegetables, legumes, whole grains, nuts, seeds, fungi, herbs and spices. Broccoli, cabbage, carrots, onions, garlic, whole wheat bread, tomatoes, grapes, cherries, strawberries, raspberries, beans, legumes, and soy foods are common sources (Moorachian, 2000).

Phytochemicals accumulate in different parts of the plants, such as in the roots, stems, leaves, flowers, fruits, or seeds7. Many phytochemicals, particularly pigment molecules, are often concentrated in the outer layers of the various plant tissues. Levels vary from plant to plant depending upon the variety, processing, cooking, and growing conditions (Ullah et al. 2019). Phytochemicals are also available in supplementary forms, but evidence is lacking that they provide the same health benefits as dietary phytochemicals (Harvey and Strategy, 2000). These compounds are known as secondary plant metabolites and have biological properties.

Such as antioxidant activity, antimicrobial effect, modulation of detoxification enzymes, stimulation of the immune system, decrease of platelet aggregation, and modulation of hormone metabolism and anticancer properties. There are more than a thousand known and many unknown phytochemicals. It is well-known that plants produce these chemicals to protect themselves, but recent research demonstrates that many phytochemicals can also protect humans against diseases (Rao, 2002).

Phytochemicals are not essential nutrients and are not required by the human body for sustaining life, but have important properties to prevent or fight some common diseases. Many of these benefits suggest a possible role for phytochemicals in the prevention and treatment of disease, Because of this property; many researchers have been performed to reveal the beneficial health effects of phytochemicals. The purpose of the present review is to provide an overview of the extremely diverse phytochemicals present in Medicinal plants (Ahmad et al., 2023).

1.2 Plants Introduction

1.2.1 Verbascum thapsus

Common mullein, also known as Wooly Mullein (Verbascum thapsus L., Scrophulariaceae) has been used as a Medicinal herb since ancient times. The leaves and flowers are reported to have expectorant and demulcent features which are used to treat respiratory problems such as bronchitis, dry coughs, whooping cough, tuberculosis, asthma, and hoarseness.

The plant is reported to be mildly diuretic to have a soothing and anti-inflammatory effect on the urinary tract, and to act as a mild sedative. It has also been used as a domestic remedy for pneumonia, fever, congestion, allergies, migraine, catarrhs, and colic (Hameed et al. 2022).

During our routine field excursions, it was found that this plant is used to treat respiratory tract infections and external boils and abscesses. Also, it has also been as a traditional remedy to treat various ailments such as spasmodic, digestive disorders, and menstrual problems. Therefore, the aim was to determine V. Thapsus extracts that have been shown earlier to have biological activity against urinary tract pathogens (Turker et al. 2002).

1.2.2 Micromeria biflora

A perennial herb, found in rock crevices and gravel slopes of sub-tropical and temperate `zones: Stem ascending, slender, 10-25cm long, glabrous. Leaves simple, small, sessile, ovate, entire, thick, acute, 2-10 mm long and 2-5mm broad. The inflorescence is verticillaster, few-flowered. Flower zygomorphic, pale pink, bract small, linear.

Calyx ribbed with narrow lobes. Corolla 2-lipped, lower lip 2-lobed, upper lip flat. Fruit outlet, oblong, brown. Flowering and Fruiting: Medicinal Value: Locally used as an herbal tea for flu, and throat irritation and plant extract treats pneumonia, stomach disorders, headache, and wounds. Essential oil is anti-inflammatory (Rajiv et al. 2016).

2. MATERIAL AND METHODS

2.1. Collection of plants and their parts

Rhizome and whole plants of Verbascum thapsus and Micromeria biflora were Collected in October 2020 from the District Lower Dir of Khyber Pakhtunkhwa.

2.2. Botanical Identification

Plant samples collected throughout the fieldwork were taxonomically identified by using Flora of Pakistan and placed in the Herbarium of Ghazi Umara Khan Degree College Samarbagh Dir Lower. The voucher specimens were kept after broad documents for future reference. From Medicinal Plant Names Services (mpns.kew.org/mpns) the correct name of the plant was confirmed.

2.2. Solvent system used

The solvents like methanol ethanol and water were used. For the preparation of crude extract of the Verbascum thapsus and Micromeria biflora plants.

2.3. Crude Extract Preparation

Plants and their parts were collected in the field and then transferred into the lab and cleaned with the help of tap water to take away the unwanted constituents and silicate material then were placed for 30 days for dryness purposes in shade at room temperature 20-20°C (Rajiv et al. 2016).

2.5. Crashing and filtration of the plants

After the dryness of the whole plants and their parts with the help of an electric grinder selected plant and their parts were ground. 10 g of plant powdered was taken then retained in a distinct conical flask and 90 ml of solvent i.e. (Methanol, Ethanol, and aqueous) was added to the plants powdered separately. Then with the help of an aluminum file, the Flask was covered and retained in a shaker for 72 hrs. for shaking purposes. After 72 hrs of shaking the extracts were filtered with the help of man filter paper and then through the filtration process plant husk was removed (Ullah et al. 2019).

2.6. Rotary evaporation of the solvents

The extract enclosed organic solvents such as Ethanol, Methanol, and water which were basically the filtrate of the particular plant and their parts. Beneath the control temperature of 30°C-35°C They were evaporated with the help of a rotary evaporator (Ullah et al. 2019).

2.7. Crud Extract

After the process of rotary evaporation certain liquid leftovers were further dried at a regulator temperature of 20°C- 25°C through a water bath. Then the plant paste was obtained known as crude extract and then the extract was placed in air-tight bottles (Fatima et al. 2023).

2.7. Phytochemical Detection

The plant extracts of Verbascum thapsus and Micromeria biflora were taken in different types of solvents i.e. Methanol, Ethanol and aqueous, and then tasted for the absence or presence of phytochemical constituents like Alkaloids, Tannins, Phlobatannins, Flavonoids, Carbohydrates, Phenols, Saponin and Glycosides (Soni et al. 2011).

2.8. Carbohydrates

The 0.5 ml of filtrate of Verbascum thapsus and Micromeria biflora was treated with 0.5 ml of Benedict’s regent and the solution was heated for 2 minutes in boiling water. Then the presence of carbohydrates was confirmed by the formation of a reddish-brown precipitate (Soni et al. 2011).

2.9. Flavonoids

The alkali substitute test was useful when the extract solution of the selected plants was treated with sodium hydroxide solution. The formation of red precipitation indicates the presence of flavonoids (Soni et al. 2011).

2.10. Phenols

2 ml of ferric chloride (FeCl₂) solution was added to 2 ml of Verbascum thapsus and Micromeria biflora

extracts solution in a test tube. Formations of deep bluish-green solution show the presence of phenol. (Soni et al. 2011).

2.11. Tannins

A ferric chloride test was done for the detection of tannins The Ferric chloride (FeCl₂) was assorted with an extract solution. The formation of blue-green coloration indicates the presence of tannins (Soni et al. 2011).

2.12. Saponins

In the test tube, five milliliters of Verbascum thapsus and Micromeria biflora plant extract were shaken dynamically. When the formation of froth occurred shown the existence of Saponins (Soni et al. 2011).

2.13. Phlobatannins

In the test tube, 0.5-gram powder of the plant extracts remained put after the addition of 2 ml water and shaken for a few minutes then the filtrate was taken and 1% aqueous HCl was added to the filtrate and then boiled in water both. The presence of Phlobatannins is indicated by the formation of a red color (Ullah et al. 2022).

2.15. Glycosides

The 5% Ferric chloride solutions and 1 ml glacial acetic acid were added to five milliliters of plant extract and then further addition of a few drops of concentered sulphuric acid. The presence of glycosides was confirmed through the formation of a greenish-blue color (Zaman et al. 2022).

2.16. Alkaloids

A few drops of Wagner’s reagent (Potassium iodine) are added to the two grams of plant extracts. When the formation of a reddish-brown precipitate occurred, it showed the presence of alkaloids (Khandelwal et al. 2015).

2.17. Tests for terpenoids

1ml of Verbascum thapsus and Micromeria biflora plant extract was assorted with 2ml of chloroform and carefully added concentrated sulphuric acid was along the sides of the tube for the formation of a layer. The formation of reddish-brown coloration indicates the presence of terpenoids (Dahiru et al. 2006).

3. RESULTS

3.1. Phytochemical analysis

A qualitative investigation of methanolic ethanolic and aqueous extracts of Verbascum thapsus and Micromeria biflora was carried out.

3.2. Qualitative Detection of Bioactive Compound Verbascum thapsus Leaves

Qualitative analysis of Verbascum thapsus and Micromeria biflora was carried out for the detection of alkaloids and flavonoids. Carbohydrates, Phlobatannins, Glycosides, Saponins, Phenol, Terpenoids, Tannins. Phytochemical detection of Verbascum thapsus plant ethanolic extracts contained a dark colour which shows that Alkaloids are present.

Where phenol was present in both methanolic, ethanolic, and aqueous extracts. The saponins in aqueous show dark concentration comparison with ethanolic and methanolic extracts.

The flavonoids were present in all three types of extract and Tannins were found in all types of extract in large amounts but in Aqueous found in small amounts. The alkaloids, glycosides, and Phlobatannins are absent in aqueous extract. The data is shown in Table 1. The highest amount of carbohydrates was present in the methanolic extract and low amounts were present in both ethanolic and aqueous extracts. In the ethanolic extracts, the glycosides were found in low amounts. In the methanolic extract, glycosides were present in the highest amount the glycosides were absent in the aqueous extract of the plant.

The Flavonoids were present in moderate amounts in both methanolic and ethanolic extract and they were present in low amounts in the aqueous extract. The Phlobatannins were present in moderate amounts in the methanolic extracts low amounts in the ethanolic extracts and absent in the aqueous extracts. The Phenol was found in all three types of plant extracts i.e. (ethanol, methanol, and Aqueous).

The Saponins were present in low amounts in the methanolic, ethanolic, and Aqueous extracts. The highest amounts of Tannins were present in the ethanolic extracts and low amounts of Tannins were found in both ethanolic and aqueous. Moderate amounts of

Terpenoids were present in the methanolic extracts of the Verbascum thapsus plants and low amounts of Terpenoids were found in both ethanolic and aqueous. The data is stated in the following Table 1.

Table 1: phytochemical detection of Verbascum thapsus leaves extracts

3.2. Qualitative Detection of Bioactive compound in the Verbascum Thapsus stems

Phytochemical detection of rhizome of Verbascum thapsus extract in all the three solvents i.e., ethanolic, methanolic, and aqueous are used which indicates the existence of bioactive compounds except glycosides are not present in aqueous extracts while the dark concentrations indicate the presence of saponins.

The qualitative phytochemical detections of the selected plants show that the Carbohydrates, Glycosides, and Tannins were present in the highest amount in ethanolic extracts followed by the methanolic extracts. The qualitative phytochemistry also shows that the Carbohydrates and glycosides were found in the highest amount in the plant extracts and the other phytochemicals such as Alkaloids, Flavonoids, and Phenol were present in moderate amounts.

The Alkaloid, Carbohydrate, Glycosides, Phlobatannins, Saponins, Terpenoids. The Alkaloids, Glycosides, and Phlobatannins were absent in Aqueous extracts. was highest in the methanolic extracts and present in low amounts in the ethanolic extracts and the alkaloids were absent in the Aqueous extracts. The flavonoids were present in all types of extracts i.e. (methanol, ethanol, and Aqueous).

Glycosides and Phlobatannins were present in the highest amount in the methanolic extract and moderate amounts in the ethanolic extracts and these both are absent in the Aqueous extracts of the P. ceritica plants. The Phenol is present in moderate amounts in the methanolic extracts and the amounts in methanolic extracts and Aqueous extracts. The saponins were present in low quantities in the P. ceritica plant.

The phytochemical Tannins were found in the highest amounts in the ethanolic extracts and moderate amounts in methanolic and Aqueous extracts. The Alkaloids, Terpenoids, Tannin, and Flavonoids are present in moderate amounts in the methanolic extracts. The data is stated in the following Table 2.

Table 2: Phytochemical detection of Verbascum thapsus stem

1 _ 2 + 2 + 3 _ 5 _ 6 + 7 + 8 + 9 + Key: -; Not found, +; Low, ++; Moderate, +++; High

3.5. Qualitative Detection of Bioactive Compound Verbascum thapsus Roots

Phytochemical detection of roots of Verbascum thapsus in all three solvents i.e., ethanolic, methanolic, and aqueous are used which indicated the existence of bioactive compounds except glycosides are not present in aqueous extracts while the dark concentrations indicate the presence of saponins.

The qualitative phytochemical detections of the selected plants show that the Carbohydrates, Glycosides, and Tannins were present in the highest amount in ethanolic extracts followed by the methanolic extracts.

The qualitative phytochemistry also shows that the Carbohydrates and glycosides, were found in the highest amount in the plant extracts and the other phytochemicals such as Alkaloids, Flavonoids, and Phenol were present in moderate amounts. The Alkaloid, Carbohydrate, Glycosides, Phlobatannins, Saponins, Terpenoids. The Alkaloids,

Glycosides, Phlobatannins, were absent in Aqueous extracts. The concentration of alkaloids was highest in the methanolic extracts and present in low amounts in the ethanolic extracts and the alkaloids were absent in the Aqueous extracts. Glycosides and Phlobatannins were present in the highest amount in the methanolic extract and moderate amounts in the ethanolic extracts and these both are absent in the Aqueous extracts of the Verbascum Thapsus plants.

The Phenol is present in moderate amounts in the methanolic extracts and the amounts in methanolic extracts and Aqueous extracts. The saponins were present in low quantities in the Verbascum Thapsus plant. The phytochemical Tannins were found in the highest amounts in the ethanolic extracts and moderate amounts in methanolic and Aqueous extracts. The Alkaloids, Terpenoids, tannins, and Flavonoids are present in moderate amounts in the methanolic extracts. The data is stated in the following Table 3.

Table 3: Phytochemical Investigation of Verbascum thapsus Root Extracts

1 _ 2 + 2 + 3 _ 5 _ 6 + 7 + 8 + 9 + Key: -; Not found, +; Low, ++; Moderate, +++; High

3.6. Qualitative Detection of Bioactive compound Micromeria biflora leaves

The highest amount of carbohydrates was present in the methanolic extract and low amounts were present in both ethanolic and aqueous extracts. In the ethanolic extracts, the glycosides were found in low amounts. In the methanolic extract, glycosides were present in the highest amount the glycosides were absent in the aqueous extract of the plant.

The Flavonoids were present in moderate amounts both methanolic and ethanolic extract and they were present in low amounts in the aqueous extract. The Phlobatannins were present in moderate amounts in the methanolic extracts low amounts in the ethanolic extracts and absent in the aqueous extracts.

The Phenol was found in all three types of plant extracts i.e. (ethanol, methanol, and Aqueous). The alkaloids, Glycosides, and Phlobatannins were absent in Aqueous extracts.

The concentration of alkaloids was highest in the methanolic extracts and present in low amounts in the ethanolic extracts and the alkaloids were absent in the Aqueous extracts. Glycosides and Phlobatannins were present in the highest amounts in the methanolic extract and moderate amounts in the ethanolic extracts and these both are absent in the Aqueous extracts of the Micromeria biflora plants. The data are shown in Table 4.

3.7. Qualitative Detection of Bioactive compound Micromeria biflora Stem

The qualitative phytochemical detections of the selected plants shows that the Carbohydrate, Glycosides, Tannins were present in highest amount in ethanolic extracts and followed by the methanolic extracts. The qualitative phytochemistry also shows that the carbohydrates, Glycosides, were found in highest amount in the plants extracts and the other phytochemicals such as Alkaloids, Flavonoids and Phenol were present in moderate’s amount.

The Alkaloid, Carbohydrate, Glycosides, Phlobatannins, Saponins, Terpenoids. The alkaloids, Glycosides, and Phlobatannins, were absent The Saponins were present in low amounts in the methanolic, ethanolic, and Aqueous extracts.

The highest amounts of Tannins were present in the ethanolic extracts and low amounts of Tannins were found in both ethanolic and aqueous. Moderate amounts of Terpenoids were present in the methanolic extracts of the Micromeria biflora plants and low amounts of Terpenoids were found both ethanolic and aqueous. The data are shown in Table 5.

Table 4: Phytochemical Investigation of Micromeria biflora Leaves Extracts

Table 5: Phytochemical Investigation Micromeria biflora Stem Extracts

3.8. Qualitative Detection of Bioactive compound Micromeria biflora Roots

Phytochemical detection of Micromeria biflora plant ethanolic extracts contained dark colour which shows that Alkaloids are present. Where phenol was present in both methanolic, ethanolic and aqueous extracts. The saponins in aqueous show dark concentration comparison with ethanolic and methanolic extracts.

Where the flavonoids were present in all three types of extract and Tannins were found in all types of extract in large amount but in Aqueous found in small amounts. The alkaloid, glycosides, and Phlobatannins are absent in aqueous extract.

The flavonoids were present in all types of extracts i.e. (methanol, ethanol and Aqueous). Glycosides and Phlobatannins were present in the highest amounts in the methanolic extract and moderates amounts in the ethanolic extracts and these both are absent in the Aqueous extracts of the Micromeria biflora plants. The Phenol is present in moderates amounts in the methanolic extracts and the amounts in methanolic extracts and Aqueous extracts. The data are shown in Table 6.

In the current study ethno-botanical importance plant of Munda Khazana District is lower Dir an overall of 50 valuable plants assembled from the research region. These plants belong to different families. The dominant valuable plants and the bases of habit were herbs (22 species) followed by shrubs (8 species) and trees (10 plant species,). Complete details of the plant, local name, botanical name, habit, family, components of plant use, and ethnobotanical utilizations are as follows.

Table 6: Phytochemical Investigation Micromeria biflora Roots Extracts

Plant No: 1

Botanical name: Ajuga parviflora Benth

Family: Lamiaceae

Local name: Kauri Booti

Habit: Shrub

Part used: Leaves

Medicinal uses: Used in curing pimples, pimples, headache, stomach acidity and pimples.

Plant No: 2

Botanical name: Amaranthus virdis L.

Family: Amaranthaceae

Local name: Chorlai

Habit: Herb

Part used: Leaves

Medicinal uses: For Diuretic, lithasis, headache swelling, and used food and fooder.

Plant No: 3

Botanical name: Cannabis sativa L.

Family: Canabaceae

Local name: Bhang

Habit: Shrub

Part used: Seeds, leaves

Ethnobotanical uses: pleasant excitement and astringent.

Plant No: 4

Botanical name: Sisymbrium irio L.

Family: Brassicaceae

Local name: Genger

Habit: Shrub

Part used: Seeds

Medicinal uses: Seeds are used in dropsy.

Plant No: 5

Botanical name: Chenopodium album L.

Family: Chenopdiaceae

Local name: Sarmay

Habit: Shrub

Part used: Seeds, leaves and roots

Medicinal uses: Use as fodder, urinary problem, and worm killer.

Plant No: 6

Botanical name: Convolvulus arvensis L.

Family: Convolvulaceae

Local name: Perwathy.

Habit: Herb

Part used: Whole plant

Medicinal uses: For fuel young plants are grazed by the cattle and skin disorders.

Plant No: 7

Botanical name: Fumaria indica (Husskn.) H.N. Pugsley

Family: Fumariaceae

Local name: Shahtra

Habit: Herb

Part used: Whole plant

Medicinal uses: It is used as fodder as well as fuel. Shoots are also used in diarrhea, blood purifier, and fever.

Plant No: 8

Botanical name: Morus alba L.

Family: Moraceae

Local name: Spen Toot

Habit: Tree

Part used: Root, leaves

Medicinal uses: Fruits are edible and wood is used for furniture as well as for the treatment of throat infection.

Plant No: 9

Botanical name: Ricinus communis L.

Family: Euphorbiaceae

Local name: Arhanda

Habit: Tree

Part used: Whole plant

Medicinal uses: Leaves are purgative, poisonous and narcotic.

Plant No: 10

Botanical name: Solanum nigrum L.

Family: Solanaceae

Local name: Kaach Maacho

Habit: Shrub

Part used: Fruits and leaves

Medicinal uses: younger leaves as used for curing fever and flue cough. Dehydrated fruits are utilized for stomach ailments.

Plant No: 11

Botanical name: Withania somnifera (L.) Dunnel. Family: Solanaceae

Local name: Kotilal

Habit: Shrub

Part used: Roots

Medicinal uses: Root paste is applied in painful swellings, bleeding wounds as well as ulcers and as well used for cure of asthma.

Plant No: 12

Botanical name: Phoenix dactylifera L.

Family: Arecaceae

Local name: Khajoor

Habit: Tree

Parts used: Fruit

Medicinal uses: It is used as an aphrodisiac and tonic.

Plant No: 13

Botanical name: Calotropis procera (Willd.) R. Br. Family: Asclepiadaceae

Local name: Spulmay

Habit: Shrub

Parts used: Leaves, flowers, latex

Medicinal uses: Remove Intestinal pain, inflammation and respiratory disease.

Plant No: 14

Botanical name: Brasica compestris L.

Family: Brassicaceae

Local name: Sharrsham

Habit: Shrub

Parts used: Leaves,

Medicinal uses: Used as food and fodder. Beautification skin disease, Asthma and cough disease.

Plant No: 15

Botanical name: Dalbergia sissoo Roxb.

Family: Fabaceae

Local name: Shawa

Habit: Tree

Parts used: Whole plant

Medicinal uses: Use for fuel and fodder.

Plant No: 16

Botanical name: Melia azedirach L.

Family: Meliaceae.

Local name: Toora shandai

Habit: Tree

Parts used: Leave, fruit

Medicinal uses: Leaf extract is employed as an antimicrobial agent, blood purification as well as for animal food.

Plant No: 17

Botanical name: Acacia nilotica (L.) Deliled. Family: Mimosaceae

Local name: kikar

Habit: Tree

Parts used: Fruit and seeds

Medicinal uses: Used for fuel as well as fodder.

Plant No: 18

Botanical name: Morus nigra L.

Family: Moraceae

Local name: Toor Tooth

Habit: Tree

Parts used: Leaves, fruit

Medicinal uses: Used for cooling agent, astringent and cleaning throat.

Plant No: 19

Botanical name: Eucalyptus camaldulensis Dehnh. Family: Myrtaceae

Local name: Lachi

Habit: Tree

Parts used: Leaves

Medicinal uses: Leaves are used to prepaire Joshanda to relieve flu. Leaves are used to cure Diarrhea.

Plant No: 20

Botanical name: Psidium guajava L.

Family: Myrtaceae

Local name: Amrood

Habit: Tree

Parts used: Leaves and fruit

Medicinal uses: Used as a tonic. It is also useful to expel abdominal worms.

Plant No: 21

Botanical name: Cynodon dactylon (L.) Pers. Family: Poaceae

Local name: Kabal

Habit: Herb

Parts used: Root, leaves

Medicinal uses: Used for animal and also use for cough, dysentery and stones.

Plant No: 22

Botanical name: Citrus sinensis (L.) Osbeck Family: Rotaceae

Local name: Malta

Habit: Tree

Parts used: Fruit

Medicinal uses: It is used for an appetizer along with tonic and also given to constipating patients.

Plant No: 23

Botanical name: Datura alba Nees

Family: Solanaceae

Local name: Dhatura

Habit: Shrub

Parts used: Whole plant,

Medicinal uses: Leaves are smoked to cure asthma.

Plant No: 24

Botanical name: Vitis vinifera L

Family: Vitaceae

Local name: Angoor

Habit: Shrub

Parts used: Fruit

Medicinal uses: Fruit is used like a common tonic and laxative.

Plant No: 25

Botanical name: Ocimum basilicum L.

Family name: Lamiaceae

Local name: Kashmally

Habit: Herb

Part used: Leaves and flower

Medicinal uses: Used as a food, shelter and fodder, Antioxidant’s skin and antipyretic disease.

Plant no: 26

Botanical name: Nerium odurum L.

Family name: Apocynaceae

Local name: Gandhery

Habit: Herb

Part used: Roots and bark

Medicinal uses: Use for fodder beatification, shelter Asthma and heart attack.

Plant no.: 27

Botanical name: Plantago lanceolate L.

Family name: Plantaginaceae

Local name: Ghwa jabbi

Habit: Herb

Part used: Whole plant

Medicinal uses: Respiratory, skin, insect and infection.

Plant no.: 28

Botanical name: Debregeasia saneb F.

Family: Urticaceae.

Local Name: Karwarra.

Habit: Shrub

Parts used: Fruits leaves.

Medicinal uses: Used as fruit fodder, fuel and used for blood purification, and stomach.

Plant no.: 29

Botanical name: Sunchus aspera L

Family name: Asreraceae

Local name: Shoda pii

Habit: Herb

Part used: Whole plant

Medicinal uses: Used as food for farm animals.

Plant no.: 30

Botanical name: Trifolium vesipinatum L.

Family name: Fabaceae

Local name: Shaftal

Habit: Herb

Part used: Whole plant

Medicinal uses: It is used as food for farm animals. The seeds utilized for pimples.

Plant no.: 31

Botanical name: Populus alba L.

Family na33e: Salicaceae

Local name: Sufedad

Habit: Tree

Part used: Whole plant

Medicinal uses: Used like a fodder, timber, fuel and furniture.

Plant no.: 32

Botanical name: Narcissus Poeticus L.

Family name: Amayrlidaceae

Local name: Goli Nargas

Habit: Herb

Part used: Flowers

Medicinal uses: It is used for ornamental purposes.

Plant no.: 33

Botanical name: Diospyros kaki L.

Family name: Ebenaceae

Local name: Amlook

Habit: Tree

Part used: Fruit

Medicinal uses: Fruit is suitable for eating; Leaves are utilized as food and fuel.

Plant no.: 34

Botanical name: Galium aparine L.

Family: Rubiaceae

Local name: Jalakai

Habit: Herb

Part use: Whole plant.

Medicinal uses: Used as Antidiuretic.

Plant No: 35

Botanical Name: Allium sativa L

Family: Amaryllidaceae

Local Name: Ouaga

Habit: Herb

Parts used: Whole plant.

Medicinal Uses: Use as a food. Arteries, high blood pressure and heart diseases.

Plant no: 36

Botanical name: Morus lavaegata Wall. ex Brandis Family name: Moraceae

Local name: Shah toot

Habit: Tree

Part used: Whole plant

Medicinal uses: Fruit is edible. Leaves are utilized as food for farm animals.

Plant no.: 37

Botanical name: Ficus carica L.

Family name: Moraceae

Local name: Inzar

Habit: Tree

Part used: Fruit and leaves

Medicinal uses: It is use as fuel and fodder for the shelter. Diabetic, migraine, diarrhea

Plant no.: 38

Botanical Name: Verbascum thapsus L.

Family: Scrophulariaceae.

Local Name: Khurdug.

Habit: Herb

Parts used: Flower leaves.

Medicinal Uses: Fruits, fuel, shelter. Tuberculosis, cough, asthma.

Plant no.: 39

Botanical name: Mentha longifulia (L.) L.

Family name: Labiateae

Local name: Enally

Habit: Herb

Part used: Leaves

Medicinal uses: It is used to relieve abdominal pain and reduce gastric acidity.

Plant no.: 40

Botanical name: Monotheca buxifolia (Falc.) A. DC. Family name: Sapotaceae.

Local name: Gurgora

Habit: Tree

Part used: Whole plant

Medicinal uses: The plant is grazing through animals and the fruits are used human food. It is also used for curing Asthma and antipyretic.

Plant no.: 41

Botanical name: Myrtus communis L.

Family name: Moraceae

Local name: Manrro

Habit: Shrub

Part used: Fruit and leaves

Medicinal uses: It is used as fodders. It is also practices to cured animal respiratory and digestive disorder.

Plant no.: 42

Botanical Name: Xanthium strumarium L. Family: Asteraceae.

Local Name: Geeshy.

Habit: Herb

Parts used: Whole Plant.

Botanical name: Medacago denticulate Willd.

Medicinal Uses: Used as fodder, tonic, fuel and in digestive problems.

Plant no: 43

Botanical Name: Zizyphus Jujube Mill.

Family: Rhamaceae

Local Name: Berra

Habit: Tree

Parts used: Whole plant

Medicinal Uses: It is used as food, fuel, Shelter and also for skin disease.

Plant no: 44

Botanical name: Cedrus deodara (Roxb. ex D.Don) G.Don

Family name: Pinaceae

Local name: Diyar

Habit: Tree

Part used: Whole plant

Medicinal uses: It is used as a food as well as for the treatment of Dysentery, Diarrhea and Urinary problems.

Plant no: 45

Botanical Name: Coriandrium sativum L.

Family: Apiaceae

Local Name: Dania

Habit: Herb

Parts used: Leaves, Fruit.

Medicinal Uses: Used as food and fodder and Skin disease, Asthma, blood purifier, cardiac and respiratory disease.

Plant No: 46

Botanical Name: Deutura innoxia Mill.

Family: Solanaceae

Local Name: Batura

Habit: Herb

Parts used: Whole plant.

Medicinal Uses: Fuel and shelter, Tonic, Dysentery and diabetic disease.

Plant no: 47

Botanical Name: Papaver somniferum L.

Family: Papaveraceae.

Local Name: Doda, kash.

Habit: Shrub

Parts used: Seeds, latex.

Medicinal Uses: Used as fodder, and fuel and also utilized for Dysentery and diarrhea.

Plant no: 48

Family name: Fabaceae

Local name: Feshtary

Habit: Herb

Part used: Leaves

Medicinal uses: Used as food and for sugar control.

Plant no: 49

Botanical name: Malva neglacta Wallr

Family name: Malvaceae

Local name: Panerak

Habit: Herb

Part used: Leaves

Medicinal uses: Food and pain.

Plant no: 50

Botanical name: Teraxicum officinale (L.) Family name: Asteraceae

Local name: Zyarr guly

Habit: Herb

Part used: Roots

Medicinal uses: Roots are used in diabetes and for kidney problems.

3. DISCUSSION

In the present study qualitative investigation of methanolic ethanolic and aqueous extracts of Verbascum thapsus and Micromeria biflora was carried out. Phytochemical detection of Verbascum thapsus plant ethanolic extracts in leaves contained a dark colour which shows that Alkaloids are present. Where phenol was present in both methanolic, ethanolic, and aqueous extracts.

The saponins in aqueous show dark concentration comparison with ethanolic and methanolic extracts. The flavonoids were present in all three types of extract and Tannins were found in all types of extract in large amounts but in aqueous found in small amounts.

The alkaloids, glycosides, and Phlobatannins are absent in aqueous extract. The Flavonoids were present in moderate amounts in both methanolic and ethanolic extract in leaves and they were present in low amounts in the aqueous extract.

The Phlobatannins were present in moderate amounts in the methanolic extracts low amounts in the ethanolic extracts of Verbascum thapsus and absent in the aqueous extracts. Tannins were present in the highest amount in ethanolic extracts followed by the methanolic extracts. The qualitative phytochemistry also shows that the Carbohydrates and glycosides, were found in the highest amount in the plant extracts and the other phytochemicals such as Alkaloids, Flavonoids, and Phenol were present in moderate amounts.

The Alkaloid, Carbohydrate, Glycosides, Phlobatannins, Saponins, Terpenoids. The alkaloids, Glycosides, and Phlobatannins, were absent in the Aqueous extracts of Verbascum thapsus. The concentration of alkaloid was highest in the methanolic extracts and present in low amounts in the ethanolic extracts and the alkaloid were absent in the Aqueous extracts.

Qualitative detection of bioactive compound Verbascum thapsus Roots in all the three solvents i.e., ethanolic, methanolic and aqueous are used which indicates the existence of bioactive compounds except glycosides are not present in aqueous extracts while the dark concentrations indicate the presence of saponins.

The highest amount of carbohydrates was present in the methanolic extract of Micromeria biflora leaves and a low amount was present in both ethanolic and aqueous extracts of roots and stem of Micromeria biflora. In the ethanolic extracts, the glycosides were found in low amounts. In the methanolic extract, glycosides were present in the highest amount the glycosides were absent in the aqueous extract of the plant.

The Flavonoids were present in moderate amounts in both methanolic and ethanolic extract and they were present in low amounts in the aqueous extract. The Phlobatannins were present in moderate amounts in the methanolic extracts low amounts in the ethanolic extracts and absent in the aqueous extracts.

A wide variety of pharmacological activities are shown by different phytochemicals, which may help in protection against chronic diseases. Tannins, flavonoids, saponins, glycosides, and amino acids have anti-inflammatory and hypoglycemic activities.

Steroids and terpenoids show central nervous system (CNS) activities and analgesic properties. Because of their antimicrobial activity saponins are involved in plant defense systems (Ayoola et al. 2008). These phytochemicals showed antimicrobial activity through different mechanisms.

With proline-rich proteins, tannins have been found to form irreversible complexes (Shimada, 2006) resulting in the inhibition of cell protein synthesis. (Parekh and Chanda, 2007) reported that tannins are known to react with proteins to deliver the typical tanning effect which is essential for the treatment of ulcerated or inflamed tissues. Herbs that have tannins as their key components are astringent in nature and are used for treating intestinal disorders such as dysentery and diarrhea (Dharmananda, 2002). Tannins and their derivatives are phenolic compounds considered to be primary antioxidants or free radical scavengers (Khan et al. 2018).

These observations therefore support the use of Verbascum thapsus and Micromeria biflora in herbal cure remedies, thus suggesting that Verbascum thapsus and Micromeria biflora has the potential as a source of important bioactive molecules for the treatment and prevention of cancer. The presence of tannins in Verbascum thapsus and Micromeria biflora supports the traditional Medicinal use of this plant in the treatment of different ailments.

Alkaloid was another phytochemical constituent observed in the extract of Verbascum thapsus and Micromeria biflora. One of the most common biological properties of alkaloids is their toxicity against cells of foreign organisms. These activities have been widely studied for their potential use in the reduction and elimination of human cancer cell lines (Nobori, et al. 1993).

One of the largest groups of phytochemicals is alkaloids in plants which have amazing effects on humans and this has led to the development of powerful painkiller medications (Kam and Liew, 2002). Shown the inhibitory effect of saponins on inflamed cells. Saponin was found to be present in Verbascum thapsus and Micromeria biflora extracts and has supported the usefulness of this plant in managing inflammation.

Flavonoids and other phytochemicals show a varied range of biological activities like anti-inflammatory, antimicrobial, analgesic, anti-angionic, cytostatic, antioxidant, and anti-allergic properties (Hodek et al. 2002).

4. CONCLUSION

The local area (Dir Lower) is rich in medicinal plants and can be used for curing different diseases instead of using imported medicines. The extraction of different chemicals from the local plants can also be done to support Pakistan’s health issues and economy.

Indigenous knowledge regarding medicinal plant collection and its proper mode of administration should be collected from the local people and preserved properly. It is suggested here that this could be done through different stockholders i.e., government, research organizations NGOs, etc.

The phytochemical analysis of medicinal plants is also important and has a commercial interest in both pharmaceutical companies and research institutes for the formation of new medicines for the treatment of several diseases. Thus, we hope that the important phytochemical properties identified by our study in the local plant of War affected area of Bajaur agency, Pakistan will be helpful in copping different diseases of this particular region.

Founding Source

There is no funding source for the students to do it by themselves.

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